Electron Microscopy at the University of Wisconsin Department of Pathology and Laboratory Medicine

University of Wisconsin-Madison Department of Pathology and Laboratory Medicine

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Electron Microscopy

Accessing the Facility

Costs for Animal Research and Cultured Cells

Electron Microscopy

UW Pathology Electron Microscopy Laboratory
   William S. Middleton Memorial Veterans Hospital
   2500 Overlook Terrace, Room A36
   Madison, WI 53705
   EM facility location

Faculty Advisor
   Terry Oberley MD, PhD
   Professor of Pathology and Laboratory Medicine

The laboratory performs the following animal tissue research and ultrastructure of cultured cells support services on a fee for service basis:

•	Initial consultation on the feasibility of a proposed project
•	Tissue preparation: Fixations, staining, embedding, and sectioning with a diamond knife on an ultramicrotome, and staining of the thin sections on the grids
•	Collaborative preembedding /or postembedding immunocytochemistry projects
•	Observation and photographic film recording of images on the Hitachi H-7000 electron microscope
•	Developing of film
•	Consultation on the obtained images in the micrographs
•	Morphometric analysis

Available equipment at the facility

•	Hitachi H-7000 transmission electron microscope (under service contract)
•	Reichert Ultracut E ultramicrotome
•	Fume hood, oven, and other lab equipment needed in the preparation of tissues and cultures for EM
•	Dedicated negative scanner to digitize negatives as TIFF images which can be burned to a CD

Contact Information
   Carol A. Sattler, PhD
   Room A36 VA Hospital
   2500 Overlook Terrace
   Madison, WI 53705
   csattler@wisc.edu
   (608) 256-1901 ext. 11723

   Terry Oberley, MD, PhD, Faculty Advisor
   Room A35 VA Hospital
   2500 Overlook Terrace
   Madison, WI 53705
   toberley@wisc.edu
   (608) 256-1901 ext 11722

Hours
   7:00 AM to 3:30 PM (or as experiment dictates)

Electron Microscope

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