CONTACT INFORMATION
Office:
Room 102 Primate
Phone:
(608) 263-0058
Fax:
(608) 265-8984
Pager:
(608) 265-7000 #3595
Mailing Address:

Room 102 Primate
1220 Capitol Court
Madison, WI 53715

Igor Slukvin, MD, PhD

  • Professor

Department of Pathology and Laboratory Medicine



Education
1999 -
2003
Residency, Anatomic and Clinical Pathology, University of Wisconsin Hospital and Clinics
1996 -
1999
Postdoc, Wisconsin National Primate Research Center
1993 -
1996
Postdoc, Louisiana State University Medical Center, Shreveport, LA
1987
PhD, Kiev Institute of Pediatrics, Obstetrics, and Gynecology, Kiev, Ukraine
1984
MD, Kiev Medical Institute, Kiev, Ukraine

Research Interests

Hematopoietic development from pluripotent stem cells; de novo generation of hematopoietic stem cells

Detail:

Hematopoietic stem cell transplantation has become the standard of care for treatment of many otherwise incurable diseases such as leukemia, lymphoma and multiple myeloma. In addition, hematopoietic stem cell transplantation is used for treatment of hereditary disorders such as anemia (sickle-cell anemia, beta-thalassemia, and aplastic anemia), inborn errors of metabolism, immunodeficiencies, and autoimmune diseases. However, insufficient numbers of hematopoietic stem cells, graft failure, inadequate anti-tumor immune response after transplantation, and graft-versus- host disease remain significant limitations to the success of hematopoietic stem cell transplantation. The main focus of research in my lab is to significantly advance the clinical use of stem cells through development of novel sources of hematopoietic stem cells and mature blood cells for transplantation, transfusion and cancer immunotherapy.

    We focused on the following strategies to generate alternative sources of therapeutic blood cells:
  • Directed differentiation of human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells into the hematopoietic stem and mature blood cells.
  • Reprogramming of pluripotent stem cells and non-hematopoietic somatic cells into the blood cells.

Using these methodologies, we anticipate generating immunologically compatible hematopoietic stem and immunotherapeutic cells in large quantities. In this way we can eliminate serious complications of bone marrow transplantation such as graft-versus-host disease and transplant failure, and at the same time generate a significant anti-tumor immune response.

We use integrative approaches, including genomics, proteomics and bioinformatics, to achieve the outlined goals and to understand important cellular, and molecular events leading to blood cell development and diversification. We already developed a very efficient system for hematopoietic and endothelial differentiation of hES and iPS cells, and directed differentiation of hES cells toward red blood cells, dendritic cells, macrophages, osteoclasts, and granulocytes. In addition, we defined the major cellular pathways leading to formation of blood cells and identified several novel hematoendothelial, hematopoietic and mesenchymal progenitors. Through comparative analysis of transcriptome and engraftment properties of these novel progenitors and fetal primitive blood cells as well as employing loss-of- and gain-on-function and lienage-tracing experiments, we expect to gain fundamental insights into molecular mechanisms leading to blood cell development. These studies could ultimately revolutionize cellular therapies for blood cancer and hereditary blood disease, and can be exploited for discovery of new drugs regulating hematopoietic stem cells, as well.


Clinical Interests
General Surgical Pathology

Appointments & Positions
2010 -
Present
Associate Professor, Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI
2004 -
2010
Assistant Professor, Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI
2003 -
2004
Instructor, Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI

Selected Publications
Dias J, Gumenyuk M, Kang HJ, et al. "Generation of red blood cells from human induced pluripotent stem cells." Stem Cells Dev.. 2011;20(9):1639-47.