TRIP Lab Resources

TRIP Faculty Presentations

In Situ Hybridization, Ricardo V. Lloyd MD, PhD

Molecular Capabilities in TRIP, William M. Rehrauer, PhD

What Can Trip IHC/Tissue Imaging Lab Offer, Wei Huang, MD

Vectra (and Inform) and It's Applications, Wei Huang, MD

Video: Biomarker Co-Localization, Analysis & Quantification, Kristina A. Matkowskyj, MD, PhD & Wei Huang, MD

Video: Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quick Guides

Nuance Quick Guide
TRIP Imaging Options - Key Features

Human Cell Line Authentication Services

Human Short Tandem Repeat Analysis

Antibodies with which TRIP has Experience (list not complete)

Ac-Histone H4 DCR3 p38 MAPK
Ah Receptor E-cad(CHD1)(CD324) pp38 MAPK
AKR1B10 E-cad (Dako) p45
AKT EGFR p52
ALDH1A1 EMA p53
Ang1 eNOS P65 NFkb
Ang2 ER alpha pAKT
Ang4 ER beta pan-CK
AR EZH2 PAR-3
ARv7 F4_80 PGE2
ARG2 FBXO10 PCNA
aSM-Actin FLK-1 p-CREB
ATM FOXP3 Plk
BAFF FOXM1 PD1
Beta Catenin GD2 PRRX1
Boris(H-120) GLB1 PSA
BRAF GLUT1 PSMA
BRMS1 Glypican 1 PTEN
Btk GRB10 S100
CA9 h327me3-in the works SENP1
Cathepsin D hAng1 SIGIRR
Cathepsin K HIF1a SirT1
CD105 HIF2a Skp2
CD133 HLA DPB1 Slug
CD147 HLA DQB1 SMAD4
CD163 HLA DR SMAD7
CD20 HP1gamma SOX2
CD3 HSP70 SSAT
CD31 HSP90 SSX
CD4 IGF1 SSX2
CD44 IGF2 Syndecan 1
CD44v3 IGF1R(3C8B1) Syndecan 2
CD45 INSM1 TDP
CD56 Ki67 TFE3
CD68 LEF1 TFEB
CD8 LRP1 TGFB1
CHD8 MCL-1 TGFB2
Chromogranin A MEDI Tie-1
CK19 MITF Tie-2
CK5 MMP2 TRK-1
CK7 NFkB TUNEL
CK8 NFkB2 Twist
Cleaved Caspase-3 NKG2D VDR
Clusterin Notch 1 VEGF
c-Met Notch 3 Vimentin
c-Myc OCT 3/4 ZEB1
CRD-BP p16 ZEB2
CREB p21
CTCF p27

Vectra/Nuance Filters and Objectives

Filters (all zero pixel shift) Excitation Filter Emission Filter
Brighfield Empty NA NA
DAPI U-FUN BP 360-370 LP 420
FITC U-FBN BP 470-495 LP 510
Cy3 U-FGW BP 530-550 LP 575
Cy5 ET Cy5 49009 BP 590-650 BP 665-715
Olympus Objectives
UPlanFL N 4x
UPlanFL N 10x
UPlanFL N 20x
UPlanFL N 100x (oil)